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Cloning Kits and Ligation

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Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Description:   Gibson Assembly* HiFi 1-Step Cloning Kit, Component: GA HiFi 1 Step Master Mix (2X), 25ul,GA Positive Control (2X), 10 uL (2 Control Reactions), Positive Control: 2 reactions, Detection Range: 200 bp to 100 kb, Method: One-step, Quantity: 5 reactions
Catalog Number: 10820-608
Supplier: TELESIS BIO



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Catalog Number: 77461-262
Supplier: TELESIS BIO


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Catalog Number: 77461-256
Supplier: TELESIS BIO


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Catalog Number: 77463-576
Supplier: TELESIS BIO


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Catalog Number: ST200550
Supplier: Agilent Technologies



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Catalog Number: ST200516
Supplier: Agilent Technologies



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Description:   Cloning Competent E. coli Sampler, Transformation efficiency: 1-3 x 109 cfu/µg pUC19 DNA, Recommended host strain for cloning genes into retroviral/ lentiviral vectors, Reduced recombination of cloned DNA (recA1), Size: 4 x2 rxns(2 x 50µl)
Catalog Number: 76299-644
Supplier: New England Biolabs (NEB)



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Description:   Gibson Assembly* Ultra Kit, Applications: Single or Multiple Insert Cloning, Large Fragment Assemblies, Mutagenesis, PCR Cloning, Detection Range: 200 bp to 100 kb, Two step reactions to generate DNA with positive control, Quantity: 5 reactions (no control)
Catalog Number: 10820-618
Supplier: TELESIS BIO



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Catalog Number: 77461-260
Supplier: TELESIS BIO


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Catalog Number: 77461-272
Supplier: TELESIS BIO


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Catalog Number: 77461-268
Supplier: TELESIS BIO


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Description:   High efficiency blunt-end PCR cloning, features DNA topoisomerase I and Cre recombinase technologies. 20 reactions
Catalog Number: 99901-044
Supplier: Agilent Technologies



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Description:   Quick Blunting Kit - 100 rxns
Catalog Number: 101417-940
Supplier: New England Biolabs (NEB)


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Catalog Number: 77463-574
Supplier: TELESIS BIO


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Catalog Number: 77461-294
Supplier: TELESIS BIO


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Catalog Number: 77461-298
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