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Cloning Kits and Ligation

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Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Description:   Modifying enzyme. For ligation, mutagenesis, cDNA cloning. Concentration: 1-3 units/uL. Size: 100 units. Packaged in a tamper-evident, clear plastic sealed packet that contains the enzyme tube, its buffer(s) and a lot-specific Promega* Product Information insert. 24-month lifetime.
Catalog Number: PAM1801
Supplier: Promega Corporation



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Description:   NEBNext* Ultra* II Ligation Module, optimized to efficiently ligate DNA adaptors compatible with Illumina* sequencing to end-repaired, dA-tailed DNA fragments, Reagents Supplied: NEBNext Ultra II Ligation Master Mix, NEBNext Ligation Enhancer, Size: 96 reactions
Catalog Number: 102969-104
Supplier: New England Biolabs (NEB)


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Description:   T7 DNA Ligase
Catalog Number: 102500-174
Supplier: New England Biolabs (NEB)


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Description:   ElectroLIgAse* combines T4 DNA lIgAse and an optimized, ready-to-use 2X reaction buffer containing a proprietary lIgAtion enhancer and no PEG
Catalog Number: 102715-942
Supplier: New England Biolabs (NEB)


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Description:   Applications: Blue/white color screening of pGEM-Z and pGEM-Zf (+/-) Vectors in the presence of X-Gal and IPTG. Production of cloned inserts as single-stranded DNA from pGEM-Zf Vectors or M13. Cloning: recA-.
Catalog Number: PAP9751
Supplier: Promega Corporation



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Description:   Instant Sticky-end Ligase Master Mix
Catalog Number: 102715-944
Supplier: New England Biolabs (NEB)


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Description:   Flexi* System, Entry/Transfer, directional cloning method for protein-coding sequences, based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient to transfer protein-coding regions, Size:5 entry and 20 transfer reactions
Catalog Number: PAC8640
Supplier: Promega Corporation



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Description:   T3 DNA Ligase
Catalog Number: 102500-168
Supplier: New England Biolabs (NEB)


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Description:   Blunt/TA Ligase Master Mix
Catalog Number: 102715-938
Supplier: New England Biolabs (NEB)


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Description:   NEBuilder* HiFi DNA Assembly Master Mix, 250 reactions, used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15-80 bp), Applications: Cloning & Synthetic Biology, Genome Editing
Catalog Number: 102877-806
Supplier: New England Biolabs (NEB)


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Description:   Has both intermolecular and intramolecular RNA strand joining activity (1-3). T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA.
Catalog Number: 101386-086
Supplier: New England Biolabs (NEB)


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Description:   T4 rna ligase 2, truncated k227q - 10,000 units
Catalog Number: 101640-956
Supplier: New England Biolabs (NEB)


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Description:   200 units.
Catalog Number: 101228-194
Supplier: New England Biolabs (NEB)


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Description:   RtcB Ligase, Concentration 15 uM, Source: RtcB Ligase is expressed as a His-tagged fusion in E. Coli single stranded RNA with a 3'-phospate or 2',3'-cyclic phosphate to another RNA with a 5' -hydroxyl (1,2). Ligation requires both GTP and MnCl2, Size: 25 reactions
Catalog Number: 103307-256
Supplier: New England Biolabs (NEB)


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Description:   Gibson Assembly Master Mix
Catalog Number: 102500-054
Supplier: New England Biolabs (NEB)


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Description:   SplintR* Ligase, also known as PBCV-1 DNA Ligase or Chlorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA splinted by a complementary RNA strand, 6, 250 units
Catalog Number: 102877-584
Supplier: New England Biolabs (NEB)


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