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Cloning Kits and Ligation

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Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Description:   Applications: Blue/white color screening of pGEM-Z and pGEM-Zf (+/-) Vectors in the presence of X-Gal and IPTG. Production of cloned inserts as single-stranded DNA from pGEM-Zf Vectors or M13. Cloning: recA-.
Catalog Number: PAP9751
Supplier: Promega Corporation



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Certificate Certificates    
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Description:   Instant Sticky-end Ligase Master Mix
Catalog Number: 102715-944
Supplier: New England Biolabs (NEB)


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Description:   T3 DNA Ligase
Catalog Number: 102500-168
Supplier: New England Biolabs (NEB)


Small Business Enterprise

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Description:   Flexi* System, Entry/Transfer, directional cloning method for protein-coding sequences, based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient to transfer protein-coding regions, Size:5 entry and 20 transfer reactions
Catalog Number: PAC8640
Supplier: Promega Corporation



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Description:   Gibson Assembly Master Mix
Catalog Number: 102500-052
Supplier: New England Biolabs (NEB)


Small Business Enterprise

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Description:   30 reactions, Ligase
Catalog Number: 101227-656
Supplier: New England Biolabs (NEB)


Small Business Enterprise

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MSDS SDS Certificate Certificates    
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Description:   10,000 units.
Catalog Number: 101228-152
Supplier: New England Biolabs (NEB)


Small Business Enterprise

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Description:   Instant Sticky-end Ligase Master Mix
Catalog Number: 102715-946
Supplier: New England Biolabs (NEB)


Small Business Enterprise

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Description:   5,000 units.
Catalog Number: 101228-188
Supplier: New England Biolabs (NEB)


Small Business Enterprise

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Description:   1,000 units.
Catalog Number: 101228-192
Supplier: New England Biolabs (NEB)


Small Business Enterprise

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Description:   SplintR* Ligase, also known as PBCV-1 DNA Ligase or Chlorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA splinted by a complementary RNA strand, 1, 250 units
Catalog Number: 102877-586
Supplier: New England Biolabs (NEB)


Small Business Enterprise

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Description:   NEBNext* Ultra* II Ligation Module, optimized to efficiently ligate DNA adaptors compatible with Illumina* sequencing to end-repaired, dA-tailed DNA fragments, Reagents Supplied: NEBNext Ultra II Ligation Master Mix, NEBNext Ligation Enhancer, Size: 24 reactions
Catalog Number: 102969-106
Supplier: New England Biolabs (NEB)


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Catalog Number: PAM8221
Supplier: Promega Corporation



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Certificate Certificates    
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Description:   Contains C8641, Flexi* System, Entry/Transfer; A9280, Wizard* SV Gel and PCR Clean-Up System; R1901, Carboxy Flexi* Enzyme Blend; and G3780, HaloTag* Flexi* Vectors—CMV Deletion Series Sample Pack.
Catalog Number: PAG6050
Supplier: Promega Corporation



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Description:   Thermostable 5' App DNA/RNA Ligase
Catalog Number: 102715-904
Supplier: New England Biolabs (NEB)


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Description:   Blunt/TA Ligase Master Mix
Catalog Number: 102715-938
Supplier: New England Biolabs (NEB)


Small Business Enterprise

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