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Cloning Kits and Ligation

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Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Catalog Number: 76717-036
Supplier: New England Biolabs (NEB)



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Description:   T4 rna ligase 2, truncated k227q - 2,000 units
Catalog Number: 101640-958
Supplier: New England Biolabs (NEB)


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Catalog Number: 76717-054
Supplier: New England Biolabs (NEB)



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Description:   Has both intermolecular and intramolecular RNA strand joining activity (1-3). T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA.
Catalog Number: 101386-088
Supplier: New England Biolabs (NEB)


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Catalog Number: PAM8225
Supplier: Promega Corporation



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Description:   T3 DNA Ligase
Catalog Number: 102500-170
Supplier: New England Biolabs (NEB)


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Description:   Modifying enzyme. For ligation, labeling, cloning. Concentration: 9-12 units/uL. Size: 500 units. Packaged in a tamper-evident, clear plastic sealed packet that contains the enzyme tube, its buffer(s) and a lot-specific Promega* Product Information (PPI) insert. 24-month lifetime.
Catalog Number: PAM1051
Supplier: Promega Corporation



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Catalog Number: PAC8820
Supplier: Promega Corporation



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Description:   Thermostable 5' App DNA/RNA Ligase
Catalog Number: 102715-906
Supplier: New England Biolabs (NEB)


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Description:   Blunt/TA Ligase Master Mix
Catalog Number: 102715-938
Supplier: New England Biolabs (NEB)


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Description:   NEBuilder* HiFi DNA Assembly Master Mix, 250 reactions, used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15-80 bp), Applications: Cloning & Synthetic Biology, Genome Editing
Catalog Number: 102877-806
Supplier: New England Biolabs (NEB)


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Description:   Has both intermolecular and intramolecular RNA strand joining activity (1-3). T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA.
Catalog Number: 101386-086
Supplier: New England Biolabs (NEB)


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Description:   T4 rna ligase 2, truncated k227q - 10,000 units
Catalog Number: 101640-956
Supplier: New England Biolabs (NEB)


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Description:   200 units.
Catalog Number: 101228-194
Supplier: New England Biolabs (NEB)


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Description:   RtcB Ligase, Concentration 15 uM, Source: RtcB Ligase is expressed as a His-tagged fusion in E. Coli single stranded RNA with a 3'-phospate or 2',3'-cyclic phosphate to another RNA with a 5' -hydroxyl (1,2). Ligation requires both GTP and MnCl2, Size: 25 reactions
Catalog Number: 103307-256
Supplier: New England Biolabs (NEB)


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Description:   Modifying enzyme. For ligation, mutagenesis, cDNA cloning. Concentration: 1-3 units/uL. Size: 100 units. Packaged in a tamper-evident, clear plastic sealed packet that contains the enzyme tube, its buffer(s) and a lot-specific Promega* Product Information insert. 24-month lifetime.
Catalog Number: PAM1801
Supplier: Promega Corporation



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