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New Avantor® J.T.Baker® premium conductive and non-conductive robotic tips deliver superior quality and reliable performance for results you can trust.
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The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.
Description:
Has both intermolecular and intramolecular RNA strand joining activity (1-3). T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA.
Description:
Modifying enzyme. For ligation, labeling, cloning. Concentration: 9-12 units/uL. Size: 500 units. Packaged in a tamper-evident, clear plastic sealed packet that contains the enzyme tube, its buffer(s) and a lot-specific Promega* Product Information (PPI) insert. 24-month lifetime.
Description:
NEBuilder* HiFi DNA Assembly Master Mix, 250 reactions, used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15-80 bp), Applications: Cloning & Synthetic Biology, Genome Editing
Description:
Has both intermolecular and intramolecular RNA strand joining activity (1-3). T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA.
Description:
RtcB Ligase, Concentration 15 uM, Source: RtcB Ligase is expressed as a His-tagged fusion in E. Coli single stranded RNA with a 3'-phospate or 2',3'-cyclic phosphate to another RNA with a 5' -hydroxyl (1,2). Ligation requires both GTP and MnCl2, Size: 25 reactions
Description:
Modifying enzyme. For ligation, mutagenesis, cDNA cloning. Concentration: 1-3 units/uL. Size: 100 units. Packaged in a tamper-evident, clear plastic sealed packet that contains the enzyme tube, its buffer(s) and a lot-specific Promega* Product Information insert. 24-month lifetime.