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Cloning Kits and Ligation

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Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Description:   RtcB Ligase, Concentration 15 uM, Source: RtcB Ligase is expressed as a His-tagged fusion in E. Coli single stranded RNA with a 3'-phospate or 2',3'-cyclic phosphate to another RNA with a 5' -hydroxyl (1,2). Ligation requires both GTP and MnCl2, Size: 25 reactions
Catalog Number: 103307-256
Supplier: New England Biolabs (NEB)


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Description:   ig-Fusion* Cloning Kit, allows to directly clone any PCR product to any linearized expression vector at any site, Include: 5x ig-Fusion* enzyme premix, 2x PCR premix & high efficiency competent cells, Joining multiple large fragments at once, Size: 50 reactions
Catalog Number: 103753-210
Supplier: INTACT GENOMICS, INC MS



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Description:   T4 DNA Ligase, Purity: >/= 99%, Product Source: E. coli strain expressing a recombinant clone, Product Includes: T4 DNA Ligase, 10x T4 DNA Ligase Reaction Buffer, Applications: Cloning of PCR products, Next-gen library, Size: 100, 000 units (400 units/µl)
Catalog Number: 103753-180
Supplier: INTACT GENOMICS, INC MS



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Description:   ig-Fusion* Cloning Kit, allows to directly clone any PCR product to any linearized expression vector at any site, Include: 5x ig-Fusion* enzyme premix, 2x PCR premix & high efficiency competent cells, Joining multiple large fragments at once, Size: 10 reactions
Catalog Number: 103753-208
Supplier: INTACT GENOMICS, INC MS



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Catalog Number: 104013-322
Supplier: INTACT GENOMICS, INC MS



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Description:   Taq DNA Ligase, Purity: >/= 99%, Product Source: E. coli strain expressing the cloned Taq DNA ligase gene from Thermus aqauticus HB8, Product Includes: Taq DNA Ligase, 10X Taq DNA Ligase Buffer with NAD+, Size: 10, 000 units (250 µl)
Catalog Number: 103753-176
Supplier: INTACT GENOMICS, INC MS



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Catalog Number: 104013-324
Supplier: INTACT GENOMICS, INC MS



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Description:   T4 DNA Ligase, Purity: >/= 99%, Product Source: E. coli strain expressing a recombinant clone, Product Includes: T4 DNA Ligase, 10x T4 DNA Ligase Reaction Buffer, Applications: Cloning of PCR products, Next-gen library, Size: 400, 000 units (2000 units/µl)
Catalog Number: 103753-184
Supplier: INTACT GENOMICS, INC MS



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Description:   T4 DNA Ligase, Purity: >/= 99%, Product Source: E. coli strain expressing a recombinant clone, Product Includes: T4 DNA Ligase, 10x T4 DNA Ligase Reaction Buffer, Applications: Cloning of PCR products, Next-gen library, Size: 100, 000 units (2000 units/µl)
Catalog Number: 103753-182
Supplier: INTACT GENOMICS, INC MS



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Catalog Number: 104013-320
Supplier: INTACT GENOMICS, INC MS



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Description:   ig-Fusion* Cloning Kit, allows to directly clone any PCR product to any linearized expression vector at any site, Include: 5x ig-Fusion* enzyme premix, 2x PCR premix & high efficiency competent cells, Joining multiple large fragments at once, Size: 100reactions
Catalog Number: 103753-212
Supplier: INTACT GENOMICS, INC MS



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